Production of laccase enzyme by Trichoderma erinaceum

M.R.Ingle; R.L.Mishra

Volume : VI, Issue : X, October - 2016

Production of laccase enzyme by Trichoderma erinaceum

M. R. Ingle, R. L. Mishra

Abstract :

Trichoderma erinaceum was isolated from decomposing coconut coir and was screened for its ability to produce laccase enzyme. Production of extracellular laccase by T. erinaceum was carried out under submerged fermentation. The extracellular laccase produced by T. erinaceum was purified and the molecular mass was determined from the analysis of SDS–PAGE. The purified laccase enzyme showed a molecular mass of 38 kDa as estimated by SDS–PAGE. ABTS has been used as substrate for determination of laccase activity for crude enzyme as well as the pure precipitated enzyme. The purified enzyme showed almost double enzyme activity (2.286 U/L) compared to the crude form (1.429 U/L). The present work represents the first report on the ability of Trichoderma erinaceum to produce laccase enzyme.