Protective role of L–ascorbic acid on dichlorovos induced alterations in the protein contents of the fresh water bivalve, Parreysia cylindrica

R. S. Tambe; P. D. Pulate

Volume : V, Issue : IX, September - 2015

Protective role of L–ascorbic acid on dichlorovos induced alterations in the protein contents of the fresh water bivalve, Parreysia cylindrica

R. S. Tambe, P. D. Pulate

Abstract :

The increasing use of pesticides in order to improve the agricultural productivity to match the population growth rate is a global phenomenon. The use of various classes of insecticides as organophosphate, organochlorine, carbamate and pyrethroids have been increased many fold for the last 10 years. Accumulation of pesticides in lower organisms as well as in the tissues of fishes, birds and human have been recorded. Accumulated pesticides induce generation of reactive oxygen species (ROS). All the bio–molecules of cell like nucleic acids, lipids, proteins and polysaccharides are potential substrates of ROS. Many investigators reported a variety of wreckage in various metabolic processes in different species exposed to different kinds of pollutants. Toxic effects of pesticides on protein content of some aquatic animals are studied by many instigators. In order to overcome such effects the most combative source would be support with exogenous antioxidant. Ascorbic acid is an important dietary antioxidant and serves to protect against oxidative damage to macromolecules such as lipids, protein, DNA and RNA which are implicated in chronic diseases (Halliwell and Guttering, 1999: Agarwal , et.al. 2003). Ascorbic acid has potential role to reduce the activity of free–radical induced reactions (Holloway and Peterson, 1984). Therefore in present investigation attempts were made to observe whether treatment of ascorbic acid would aggravate or ameliorate the biochemical alteration in tissues induced by pesticide in model animal freshwater, bivalve. The investigation regarding the biochemical changes after pesticide exposure and its subsequent recovery in non target aquatic species such as molluscs was insufficient. Hence in the present study an attempt was made to investigate the effect of chronic treatment of pesticide dichlorovos and its subsequent recovery by exogenous administration of Lascorbic acid on the protein contents of different soft body tissues of fresh water bivalve, Parreysia cylindrica. An investigation was undertaken to evaluate the effectiveness of ascorbic acid on Dichlorovos induced alteration on the ascorbic acid levels in an experimental model, the fresh water bivalve Parreysia cylindrica. The effect on bivalve was studied under five groups. Group A was maintained as control, group B bivalves were exposed to chronic dose of C4H7CI2O4P (0.04023ppm) while group C bivalve where exposed to respective chronic concentration of Dichlorovos with 50mg/L of L– ascorbic acid for 21 days. Were divided into D and E group. The D group bivalves were allowed to cure naturally while E group bivalves were exposed to 50mg/L of L–ascorbic acid for recovery. After every seven days bivalves from A, B, C, D & E were removed, and their tissue were separated and dried at 80 0c .From each powder, ascorbic acid level was estimated and presented as mg/gm of dry weight. Significant decrease was observed in ascorbic acid content on the exposure to Dichlorovos. However; depletion in the L–ascorbic acid level on the exposure to dichlorovos with L–ascorbic acid was minimum. Pre–exposed bivalves to dichlorovos showed fast recovery in the ascorbic acid exposed bivalves than those, which were allowed to recover naturally; the probable role of L– Ascorbic acid is discussed in the paper.