Volume : IV, Issue : XII, December - 2014

Purification of an Alkaline Protease Suited for Ecofriendly Sanitation in Milk Processing Units

Beena, A. K

Abstract :

The objective of this work was to purify an alkaline protease suited for ecofriendly sanitation in milk processing units from bacteria of milk origin.Bacillus cereus isolated from sterilized skim milk was used for the production of protease enzyme.The crude protease obtained in this work performed maximally at pH 11.0. Enzyme retained 92.6 per cent of the original activity after 24 h of exposure to pH 12. Increased activity under alkaline conditions is suggestive of alkaline nature of enzymes.Qualitative assessment ofthe cleaning efficiency on proteinaceous soil confirmed the superiority of enzyme based formulation when compared to control.A purification fold of 141.31 was attained by ammonium sulphate fractionation, dialysis and column chromatography. SDS polyacrylamide gel electrophoresis under reducing conditions revealed a single band indicating that theenzyme preparation is homogenous.Based on Mf values, molecular weight of enzyme obtained in this study was approximated as 50.5 kDa.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

BEENA, A. K Purification of an Alkaline Protease Suited for Ecofriendly Sanitation in Milk Processing Units Indian Journal of Applied Research, Vol.4, Issue : 12 December 2014

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