PYOCYANIN EXERTS ANTIMICROBIAL ACTIVITY ON STAPHYLOCOCCI

DR ARIJIT CHATTERJEE

Volume : V, Issue : X, October - 2015

PYOCYANIN EXERTS ANTIMICROBIAL ACTIVITY ON STAPHYLOCOCCI

Dr Arijit Chatterjee

Abstract :

Different isolates of Pseudomonas aeruginosa were obtained from wound swab, pus and urine specimens. The isolates were cultured for the production of the blue pigment; pyocyanin. Considerable amounts of blue pigment were produced by P. aeruginosa isolates when grown on nutrient agar media. Müller–Hinton agar was further used for growth, pigment production and sensitivity tests. P.aeruginosa isolates were grown in peptone water liquid medium by incubating at 370C. for 24hrs. Then, culture tubes were shaken gently and centrifuged 10000rpm for 30 minutes to extract the supernatant. Using 5mL of the bacterial supernatant, 1mL of chloroform was added and shaken vigorously. The chloroform layer was removed (which should be on the bottom and turn blue to contain the pyocyanin. 0.5mL of 0.01M HCl was added and the aqueous layer was removed which becomes red in colour. 5 L of 10nM NaOH was added again to revert the colour to blue and thus purified. The growth of Staphylococcus aureus was completely inhibited when cultivated on the agar plates containing the blue pigment.