Rapid phenotypic detection and differentiation of pathogenic aerobic bacteria using alternative methods to staining.

Vinithra Suresh; Kousalya M; Nachammai SM

Volume : VIII, Issue : I, January - 2018

Rapid phenotypic detection and differentiation of pathogenic aerobic bacteria using alternative methods to staining.

Vinithra Suresh, Kousalya M, Nachammai Sm

Abstract :

Background & aim: The present study investigates the pathogenic aerobic bacteria isolated from different sources of clinical samples in a tertiary care hospital with the aim in proving that there are also couple of other methods for confirming the preliminary classification of bacteria without using Gram staining.

Materials & methods: The study has been carried out during December 2015 to March 2016. All the isolates were subjected to Gram staining, Vancomycin susceptibility test and KOH string test.

Results: Our prospective study showed that out of 268 isolates, 185 (69.02%) were gram negative bacilli and 83(30.97%) were gram positive cocci. Gram negative rods including Escherichia coli, Klebsiella sp., Pseudomonas sp., Proteus sp., Enterobacter spp. and gram positive cocci included Staphylococcus sp., Streptococcus sp. and Enterococcus sp. All gram negative bacilli shows 100% vancomycin resistance and 100% KOH test positive. On the other hand, all gram positive cocci show 89.15% vancomycin sensitive, 10.84% resistance and KOH string test is 100% negative.

Conclusion: The alternative test of Gram staining is Vancomycin susceptibility test and KOH test that easily identifies the gram negative and gram positive bacteria very well.