Relative variability in transformational efficiency of  CaCl2, MgCl2, Tris–HCl, and Tris EDTA in E coli and  Agrobacterium tumefaciens

Ms. Mrinalini; Mr. P. Rathinasabapathi; Mr. Rex Arunraj; Mr. A. Muralidharan

Volume : III, Issue : V, May - 2013

Relative variability in transformational efficiency of CaCl2, MgCl2, Tris–HCl, and Tris EDTA in E coli and Agrobacterium tumefaciens

Ms. Mrinalini, Mr. P. Rathinasabapathi, Mr. Rex Arunraj, Mr. A. Muralidharan

Abstract :

Bacterial transformation is an inevitable tool for gene manipulation and plant transformation. Ca2+ divalent cations are indispensable for the induction of competency in E coli transformation. Different types of ions have been reported to induce and enhance transformation in E coli with varied efficiency. In the current study we investigated the effect of CaCl2, MgCl2, Tris–HCl, Tris EDTA, NaOAc and NH4OAc in Escherichia coli and Agrobacterium tumefaciens. The results indicate that the effect of chemicals on E coli and agrobacterium may depend on the electrochemical physiology of the cell and the memane characteristics. It is reported in the current study that the efficiency of mediator chemicals are appreciably varying when related to the two samples.