Resistant Pattern and Molecular Characterization  of Extended–Spectrum ?–Lactamse–Producing  Escherichia Coli in Skaka/ Saudi Arabia

Omer M. Khalil; Ali E. N; Baraka H. Saleh

Volume : III, Issue : VI, June - 2013

Resistant Pattern and Molecular Characterization of Extended–Spectrum ?–Lactamse–Producing Escherichia Coli in Skaka/ Saudi Arabia

Omer M. Khalil, Ali E. N, Baraka H. Saleh

Abstract :

In this study 82 isolates of E.coli were identified and subjected to antibacterial activity against 18 different antibiotics.PCR analysis was used to isolate five ESBL genesandto confirm the identification of?–lactamases producers observed during Kirby–Bauers disc diffusion method using indicator antibiotics.PCR identified the presence of CTX–M, TEM, SHV, CTX–M +TEM, CTX–M+SHV at the following sequences 39.0%, 18.3%, 12.2%, 6.2% and 1.2% respectively. 23.2% of the ESBL isolates did not express any of the above resistance genes. All the ESBL producing Escherichia colistrains were resistant to ampicillin, cefazolin, ceftazidime, ceftriaxone and cefepime while imipenem,meropenem and amikacin were highly active in all the isolates, the other antibiotics expressed different degree of activity as shown in table (3) .This result confirmed the existence of high level of multi drug resistance( MDR) withinthe extended–spectrum ?–lactamase Escherichia coli in Skaka and the predominance of CTX–M gene.