ROLE OF Citrullus colocynthis Schard ON ROTENONE INDUCED BRADYKINESIA IN EXPERIMENTAL RATS

Tausif Ahmed; Shivaprasad Hudeda; Manjula SN

Volume : VII, Issue : X, October - 2017

ROLE OF Citrullus colocynthis Schard ON ROTENONE INDUCED BRADYKINESIA IN EXPERIMENTAL RATS

Tausif Ahmed, Shivaprasad Hudeda, Manjula Sn

Abstract :

The current study aims at investigating the effect of INDRAVARUNI – Citrullus colocynthis Schard on rotenone induced adykinesia in experimental rats. 42 healthy male Wistar rats were selected for the study and were divided into 7 groups. Before inducing Parkinson’s disease, the experimental animals were acclimatized for three days. The Rotenone solution was administered at 2.5mg/kg body weight/day intraperitonealy to all the groups, except for the normal control group which received only the vehicle (DMSO + Triester F 810). The respective treatment groups received their treatment from the day of starting of rotenone injection. Animals developing debilitating phenotype – limiting their mobility, feeding or grooming; were sacrificed and ain samples procured from them. Otherwise, the animals were assessed for their motor scores using Open field Test in three sessions – Day 0 (before induction), Day 2^nd & Day 5^th.

Ultimately the rats surviving till the 9^th day were also sacrificed on 10^th day and ain samples procured, which were used for further analysis. One sample from each group was analysed for striatal dopamine levels using HPLC, as a supportive observational study. The BID and TDS dosage groups showed maximum efficacy in preventing adykinesia.