Screening and Characterization of Xenobiotic (Phenol) Degrading Bacteria with Reference to Bioremediation

Dr Pragya Rathore; Kritika Sinha

Volume : III, Issue : VII, July - 2014

Screening and Characterization of Xenobiotic (Phenol) Degrading Bacteria with Reference to Bioremediation

Dr Pragya Rathore, Kritika Sinha

Abstract :

Microorganisms play key role for saving the environment by degrading various chemical compounds which are toxic in their native forms or may be modified either. Removal of such environment polluting compounds by microorganisms can be proved to be contemplatable. Because of their degradability, they are often found in the areas affected with such chemicals. And so the organisms can be isolated from the area. In the present study, we have focused for the degradation of the xenobiotic compounds and the organisms were isolated from the soil contaminated with oil by the enrichment technique using phenol as sole carbon and energy source, purified and checked for their ability to degrade phenol. The isolates were subjected to biochemical characterization at the genus level and according to Bergey’s Manual of Determinative Bacteriology, the isolates characterized were Staphylococcus, Bacillus, Pseudomonas, Micrococcus, Streptococcus. Out of all the isolates, the third isolate Pseudomonas showed the best results for the phenol–degrading capability