Volume : IV, Issue : XII, December - 2014

SDS–PAGE Analysis of Proteins in Sterile and Fertile Anthers in Sorghum.

R. D. Borse

Abstract :

The present investigation employing Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDSPAGE), analysis of protein profiles of fertile and sterile lines of sorghum. Polyacrylamide gel were analyzed on their protein banding patterns (profiles). The A line (male sterile) differed significantly with B (male fertile) regarding composition of certain bands. Molecular weights of these proteins were determined employing the protein molecular weight marker, PMW–M of Genei, Bangalore, India Ltd. Difference in polypeptide profiles has been shown between male–sterile and fertile anthers. Whether the lack of a number of other polypeptides is directly responsible for pollen degeneration. Results indicate that the fertile and sterile anthers differed in their polypeptide band composition.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

R.D. BORSE SDS-PAGE Analysis of Proteins in Sterile and Fertile Anthers in Sorghum. Indian Journal of Applied Research, Vol.4, Issue : 12 December 2014

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