Volume : VII, Issue : X, October - 2017

Significance of measuring Anti–HBc assays in detecting occult Hepatitis B infection in donor samples – An Indian study.

Dr Satish Kumar, Dr Umesh Kapoor, Dr Alpana Gupta, Dr Amit Pawar, Dr Ujjwal Dimri

Abstract :

 Introduction

It has been observed that Hepatitis B transmission can occur from HBsAg negative blood donors despite adopting highly sensitive screening measures. Thus, the term occult hepatitis B virus infection was introduced.

Material and Methods

6000 healthy donor samples were screened for Hepatitis B infection using Hepatitis B markers viz., HBsAg, Total anti–HBc. The percentage of Occult HBV infection with IgM anti–HBc and/or total anti–HBc antibody alone was calculated. Samples which were total anti–HBc reactive and anti–HBs negative (< 12 mIU/mL) were considered Isolated anti–HBc.

Result and Discussion

A total 500 samples were tested positive for total Anti–HBc (8.3%) while out of these 39 samples were also HBsAg positive. The prevalence of HBsAg in this study is 0.65%. Among 461 samples which were anti–HBc positive were subjected anti–HBs. 80 samples were anti–HBs negative. The prevalance of "Isolated anti– HBc" in healthy donors in this study was 1.33% and prevalence of Occult HBV infection was (19 out of 6000) i.e. 0.316 %.

 

 

 

Conclusion

Anti–HBc and nucleic acid testing in transfusion settings can be minimized by the use of very sensitive HBsAg screening assays to reduce the false positive OBI. 

Keywords :

Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Dr Satish Kumar, Dr Umesh Kapoor, Dr Alpana Gupta, Dr Amit Pawar, Dr Ujjwal Dimri, Significance of measuring Anti–HBc assays in detecting occult Hepatitis B infection in donor samples – An Indian study., INDIAN JOURNAL OF APPLIED RESEARCH : Volume-7 | Issue-10 | October-2017

Number of Downloads : 551

References :