Volume : IV, Issue : X, October - 2014

SNP Screening of PC–1 Gene And Biochemical Analysis In Type–II Diabetic Patients From Jaipur (Rajasthan, India)

Jitendra Kumar Sharma, Mohan Lal Bansal, Parveen Kumar Vats, Ranjeet Singh, Jainder Singh Chhilar,

Abstract :

The type II diabetes mellitus (T2DM) is characterized by insulin resistance but the genes responsible for it are poorly defined. Ectonucleotide pyrophosphatase–1 (ENPP1) also known as plasma cell memane glycoprotein–1 (PC–1) is known to influence insulin signal transduction pathway. Previous studies have demonstrated that the K121Q variant of ENPP1 gene has a significant role in determining susceptibility to insulin resistance resulting in T2DM. To assess whether the K121Q polymorphism in ENPP1 gene has any impact on T2DM and other atherogenic factors in Indian subjects, a case–control association study was done on 28 T2DM and 27 control subjects from Jaipur, Rajasthan, India. Blood samples were collected under fasting condition to assess the relevant biochemical parameters. PCR–RFLP analysis revealed the KK, KQ and QQ genotypic frequencies (at the 121 position of the ENPP1) to be 71.4%, 28.6%, 0% and 70.4%, 29.6%, 0% in diabetic and control subjects, respectively. However between T2DM and control subjects fasting blood glucose (FBG) was found to vary significantly within and between different genotypic groups. Clinical parameters and atherogenic factors among genotypes were found to have no significant differences. There was significant positive correlation between systolic and diastolic blood pressure– 0.707**, total cholesterol (TC) and triglycerides 0.354**, TC and high density lipoprotein (HDL–C) 0.407**, TC and low density lipoprotein (LDL–C) 0.958** and TC and very low density lipoprotein (VLDL–C) 0.354** and HDL–C and LDL–C 0.226* while there was nonsignificant negative correlation between BMI and FBG –0.084. χ2 –test revealed significant association of genotypes with FBG (χ2– value 9.655, df= 3, sig= 0.022). We conclude that the K121Q polymorphism in the present population from Jaipur, Rajasthan, India is moderate ranging from 25–30% that is having significant positive correlation with FBG.

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Jitendra Kumar Sharma, Mohan Lal Bansal, Parveen Kumar Vats, Ranjeet Singh, Jainder Singh Chhilar, Snp Screening of Pc-1 Gene And Biochemical Analysis In Type-Ii Diabetic Patients From Jaipur (Rajasthan, India) Indian Journal of Applied Research, Vol.4, Issue : 10 October 2014

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