IJAR - Indian Journal of Applied Research

Mohammed Abu El-Magd; Haitham G. Abo-Al-Ela; Abeer F. El-Nahas and Ali A. Mansour

Volume : III, Issue : XII, December - 2013

SNPs of the MyoD and MyoG genes and their association with growth traits in Egyptian water buffalo (Bubalus bubalis)

Mohammed Abu El Magd, Haitham G. Abo Al Ela, Abeer F. El Nahas And Ali A. Mansour

Abstract :

Myogenic determination gene (MyoD) and Myogenin (MyoG) are members of the myogenic regulatory factors (MRF) gene family which have great role in the development of skeletal muscle. Association between the polymorphisms of MyoD and MyoG genes and growth traits were studied in many species but have not elucidated yet in buffalo. Therefore, the aim of this study was to detect polymorphisms of these two genes, their allele, and genotype frequencies and to determine associations between these polymorphisms and growth traits in Egyptian water buffalo. Four loci of the MyoD gene and five loci of MyoG gene were amplified by PCR and subsequently, subjected to single strand conformation polymorphism (SSCP) followed by sequence analysis to identify different allelic patterns. A total of 4 novel single nucleotide polymorphisms (SNPs) were detected among Egyptian water buffaloes; A1481C SNP in intron 2, C1876T SNP in 3/UTR of MyoD and T1198C in intron 1, C2858T in 3/UTR of MyoG. Moreover, 23 SNPs in MyoD were determined in Egyptian water buffaloes as compared to Italian buffalo (FJ194946), and 43 SNPs in MyoG compared with water buffaloes in China (EF636460). The association of these polymorphisms with growth traits in Egyptian buffalo was analyzed using the general linear model (GLM) which revealed no significant association of any detected SNP with the measured growth traits from birth to 24 mo of age. Thus, these 4 SNPs may not influence growth traits in Egyptian buffalos.