Spectroscopic evaluation of enterococcus faecalis, and streptococcus species in secondary root canal infection of  type–2 diabetic patients

Dr.Grishma Florence Noronha; Prof:Dr. Moksha nayak; Dr. Ramya M.K; Dr.JayanthNambiar; Dr.Moopilmidhunmohanan

Volume : V, Issue : VII, July - 2015

Spectroscopic evaluation of enterococcus faecalis, and streptococcus species in secondary root canal infection of type–2 diabetic patients

Dr. Grishma Florence Noronha, Prof Dr. Moksha Nayak, Dr. Ramya M. K, Dr. Jayanthnambiar, Dr. Moopilmidhunmohanan

Abstract :

Aim:– Detection and Identification of Enterococcus faecalisand Streptococcus species in secondary root canal infections of non diabetic and type–2 diabetic patients using spectroscopic signature development and evaluation. Material and methods:40 subjects grouped into 20 non diabetic and 20 type–2 diabetic subjects with secondary root canal infection were selected for the study. Root canal samples were collected and cultured using selective media to isolate Enterococcusfaecalis and Streptococcus mitis. The microorganisms obtained were subcultured, washed, and resuspended in phosphate buffered saline and directly analyzed using Transmission Fourier transform infrared spectroscopy (FTIR). The spectra obtained were correlated with reference spectrum liary Statistical analysis was done using Student unpaired t test, Pearson’s Chi–Square test and Fischer’s exact test. Result: Evaluation by FTIR spectroscopy detected type–2 diabetic subjects (85%) was associated with almost twice the amount of microorganism compared to non diabetic (45%). Enterococcus faecalis is the most predominant microorganism both in non diabetic(25.0%) and type–2 diabetic subjects (45.0%) followed by Streptococcus spp20.0% and 30.0% respectively. A strong association was noted between Enterococcus faecalisand Streptococcus mitisin non diabetic subjects (p<0.05) Conclusion:Enterococcusfaecalis, and Streptococcus sppwas significantly associated with type–2 diabetic subjects as identified usingFTIR. FTIR proved to be a reliable rapid and economic assessment tool for evaluation of endodontic pathogens.