Spectrum of Extrapulmonary Tuberculosis in a Tertiary Care Hospital in Western Uttar Pradesh

Saumya Srivastava; Vandana Sardana; Anita Pandey

Volume : VIII, Issue : IX, September - 2018

Spectrum of Extrapulmonary Tuberculosis in a Tertiary Care Hospital in Western Uttar Pradesh

Saumya Srivastava, Vandana Sardana, Anita Pandey

Abstract :

Aims & Objectives– i)To determine the frequency of extra–pulmonary tuberculosisin clinically suspected cases ii)To evaluate the efficacy of Polymerase Chain Reaction (PCR)as a rapid and sensitive diagnostic tool in comparison to gold standard (i.e. culture).Method–A total of ninety seven samples, collected from clinically suspected cases of extra–pulmonary tuberculosis, were processed for detection of Mycobacterium tuberculosis by direct microscopy (ZN staining), automated liquid culture method (BacT/ALERT 3D system)and PCR assay. Results– The frequency of EPTB cases in the present study was 11.3% (11/97). The most common site was endometrial tissue (5/11, 45.4%), followed by pleural fluid (4/11, 36.4%), and lymph node aspirate and fallopian tube (each 9.1%). Amongst the positive cases, predominant age group was 21–30 years, and females outnumbered males with a ratio of 4.5:1.The positivity rate of PCR was 11.3% (11/97) as compared tothat of culture and smearthat is 7.22% each respectively (7/97). The sensitivity,specificity, PPV and NPV of PCR was 100%, 95.6%, 63.6% and 100% respectively as compared to culture. Conclusion: PCR assay targeting 123 bp fragment of IS6110 is a rapid, sensitive and specific method for the early diagnosis of extra–pulmonary tuberculosis, especially in samples which are pauci–bacillary in nature.