STAT6 PROBABLY INHIBITS TRANSCRIPTION FACTOR PAX-5 VIA ID2 AND E2A IN THE PATHOGENESIS OF ALLERGIC BRONCHIAL ASTHMA

Mineev Valeriy Nickolaevich; Nyoma Mikhail Alexandrovich; Sorokina Lada Nickolaevna

Volume : VIII, Issue : II, February - 2018

STAT6 PROBABLY INHIBITS TRANSCRIPTION FACTOR PAX-5 VIA ID2 AND E2A IN THE PATHOGENESIS OF ALLERGIC BRONCHIAL ASTHMA

Mineev Valeriy Nickolaevich, Nyoma Mikhail Alexandrovich, Sorokina Lada Nickolaevna

Abstract :

Background. Asthma pathogenesis is known to be based on cytokine and transcription factors activities. Putative interactions between two B–lymphocyte transcription factors STAT6 and PAX–5 was analyzed.

Methods. Peripheral blood lymphocytes from allergic, non–allergic asthma patients and healthy persons were used. RT–PCR was performed to evaluate PAX–5 mRNA levels. STAT6 and pSTAT6 levels were analyzed using western blotting.

Result. РАХ–5 mRNA expression in ABA with normal IgE levels correlated with STAT6 mRNA levels. In ABA, effects of IL–4 caused a more significant decrease of РАХ–5 mRNA compared with healthy persons. Negative correlation between РАХ–5 mRNA expression and STAT6 levels shown in high levels of mRNA РАХ–5 and low levels of STAT6 groups.

Conclusion. The discovered associations between STAT6 levels and РАХ–5 mRNA expression levels suggest that STAT6 may negatively influence PAX–5 transcription in ABA patients, thus, probably, promoting differentiation of B–cells into plasma cells.