Strain Improvement of Mycophenolic Acid Producer Penicillium Brevicompactum Through Mutagenesis

Umesh Luthra; Aditi Trivedi; Sneha Khadpekar

Volume : IV, Issue : X, October - 2014

Strain Improvement of Mycophenolic Acid Producer Penicillium Brevicompactum Through Mutagenesis

Umesh Luthra, Aditi Trivedi, Sneha Khadpekar

Abstract :

Mycophenolic acid is a immunosuppressive drug, produced by Penicillium evicompactum, having various other biological properties. Strain improvement of Penicillium evicompactum was carried out by ultra violet radiation (UV), ethyl methane sulphonate and methyl viologen. The survival percentage of the spores was estimated for different period of exposure of the mutagens. Two mutant colonies were obtained by the mutagenesis experiments. The colonies P120EMS1 and P90MV2 were derived from ethyl methane sulphonate and methyl viologen treatment respectively. The yield varied from mutant to mutant with maximum yield of 10.942mg/g produced by methyl viologen mutant P90MV2 followed by P120EMS1 produced by ethyl methane sulphate mutation giving 10.216 mg/g. Comparative analysis revealed that MV showed an increase in MPA yield by 28 % whereas ethyl methane sulphonate and UV radiation showed an increase of 20 % and 5 % respectively. Additionally, the hereditary stability stud