Studies on Applicability of Alkaline Protease  from Halophilic Bacteria in Detergent Formulations

Vinod Kumar Nigam; Archana Vimal; Ambarish Sharan Vidyarthi; Medicherla Krishna Mohan

Volume : III, Issue : IX, September - 2013

Studies on Applicability of Alkaline Protease from Halophilic Bacteria in Detergent Formulations

Vinod Kumar Nigam, Archana Vimal, Ambarish Sharan Vidyarthi, Medicherla Krishna Mohan

Abstract :

Alkaline proteases are one of the major groups of industrial hydrolytic enzymes used in detergents formulations to clean various proteinaceous stains like blood, egg, milk, vegetable gravy as well as proteins from body secretions. The compatibility of crude alkaline proteases produced from halophilic Bacillus sp. have been evaluated in presence of different commercial detergents (Ariel, Tide, Wheel, Surf Excel and Ujala techno–ight etc.), surfactants, bleaching agents etc. at 50 oC and 60 oC respectively and it was found that the enzyme is stable in detergents (87–105% of residual activity), surfactants like Triton X–100 and Tween–80 (up to 1%), cetyltrimethylammonium omide (5 mM) and bleaching agent sodium hypochlorite (0.2%), while 47% of residual activity was observed in presence of 0.5% sodium dodecyl sulfate. The maximum alkaline protease activity was observed at 50 oC and at a oad pH range of 8.0 to 10.0.