Study Of Antibacterial Activity Of β–Sitosterol Isolated From The Leaves Of Nyctanthes Arbor Tristis

Dr.Subhadra Rajpoot; Dr.Preeti Singh

Volume : IV, Issue : IX, September - 2014

Study Of Antibacterial Activity Of β–Sitosterol Isolated From The Leaves Of Nyctanthes Arbor Tristis

Dr. Subhadra Rajpoot, Dr. Preeti Singh

Abstract :

Nyctanthes arbortristis Linn. belonging to family Oleaceae is a well known medicinal plant.The stem bark extracts of the plant were tested for their in vitro antimicrobial activity by cup plate method. The test organisms were Staphylococcus aureus, Micrococcus luteus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger. The zone of inhibition and Minimum Inhibitory Concentration (MIC) of the extracts were determined and compared with the standard drugs ciprofloxacin and fluconazole. The chloroform extract was found to have both antibacterial and antifungal activity whereas the petroleum ether and ethanol extracts possess only antibacterial activity. The antibacterial potentialof a sitosterol isolated from the ethanolic extract of leaves of Nyctanthes arbortristis was evaluated on gram–positive (Staphylococcus aureus) and gram–negative (Escherichia Coli,Pseudomonas aeruginosa) bacteria.The compound was analysed for its antibacterial potential in terms of zone of inhibition of bacterial growth and showed the significant antibacterial activity against gram–negative bacteria.