Study of Association of Oxidative Stress, C-reactive protein and Liver Enzymes in Relation to Chronic Liver Disease.

Dr. Madhumita Chatterjee; Faruque Ahmed; Dr.Shruti Priya

Volume : VIII, Issue : XII, December - 2018

Study of Association of Oxidative Stress, C-reactive protein and Liver Enzymes in Relation to Chronic Liver Disease.

Dr. Madhumita Chatterjee, Faruque Ahmed, Dr. Shruti Priya

Abstract :

Background : Oxidative stress is a prominent feature in the pathophysiology of both acute and chronic liver disease. Oxidative stress occurs when reactive oxygen species generated from either exogenous sources (e.g. alcohol and acetaminophen) or endogenous sources (e.g. Liver cytochrom P₄₅₀ enzyme system) and cellular functions (mitochondrial metabolism).Oxidative stress is increasingly recognized as an important factor in the progression of chronic liver diseases of varying etiologies.

Aim: To evaluate oxidative stress status through measurement of Malondialdehyde (MDA) and to analyze association of changes in MDA status with respect to fluctuations in inflammatory marker (CRP) and liver enzymes in CLD.

Materials and Methods: The study included 50 Chronic Liver Disease (CLD) cases and 50 age and sex matched controls. Plasma MDA was measured by spectrophotometric method (TBARS) at 531nm, and biochemical parameters like serum ALP, AST, ALT were measured by autoanalyzer( Turbochem–100,model No–4600). Estimation of C–Reactive Protein was done by RHELAX–CRP Slide test on the basis of principle of agglutination.

Result– The MDA and CRP levels were increased in CLD cases along with liver enzymes and were statistically significant, There is positive correlation of MDA, CRP with severity of liver disease.

Conclusion: It is concluded that MDA, CRP and the transaminases ALT, AST, and ALP levels are higher in the studied liver pathologies as compared to control values