Study of cyclophilin� A & hscrp  as predictors biomarkers  in patients with type 2 diabetes mellitus and Suggests presence of vascular diseases

MM Shah; Dr Z.G. Badade; Dr. S.K. kaul

Volume : VI, Issue : XI, November - 2016

Study of cyclophilin� A & hscrp as predictors biomarkers in patients with type 2 diabetes mellitus and Suggests presence of vascular diseases

Mm Shah, Dr Z. G. Badade, Dr. S. K. Kaul

Abstract :

Aims/hypothesis: Cyclophilin A, an immunophilin is secreted from human monocytes activated by high glucose. its role as an inflammatory mediator of vascular tissue damage associated with inflammation and oxidative stress, we examined plasma levels of cyclophilin A in normal healthy volunteers and patients with type 2 diabetes(DM), with or without coronary artery disease (CAD). Methods: Study subjects comprised of 30 normal healthy volunteers, 30 patients with DM and CAD, 30 patients Non Diabetic with CAD, 30 patients with diabetes. Diabetes was assessed by HbA1c levels while coronary artery disease was established coronary angiography. Plasma cyclophilin was measured using a cyclophilin A ELISA Kit. Relationship of plasma cyclophilin A levels with blood markers of type 2 diabetes. hsCRP is done by ELISA Kit(immuno–inhibition method). Results: Plasma Cyclophilin levels were higher in diabetes patients with or without CAD compared to normal subjects (P < 0.001). Fasting blood sugar levels and HbA1C levels were positively associated with increased plasma cyclophilin. Increased level of hsCRP shows systemic inflammation in study group. Conclusions/interpretations: Our study shows that patients with type 2 diabetes have higher circulating levels of cyclophilin A than the normal Healthy controls. Plasma cyclophilin levels were increased in patients with diabetes and coronary artery disease suggesting a role of this protein in accelerating vascular disease in type 2 diabetes. Considering the evidence that Cyclophilin A is an inflammatory mediator in atherogenesis. hsCRP levels are significantly higher in study groups as compared to healthy individuals.