Volume : IX, Issue : I, January - 2019

Study of Enzyme activity for resveratrol production by endophytic fungus Aspergillus niger VVE1

Chetana Roat, Meenu Saraf

Abstract :

Trass–resveratrol is a polyphenolic compound belonging to the group of stilbenes. The compound has gained increasing interest over the last few years. Resveratrol synthesized via phenypropanoid pathway.The aim of present study was to establish an enzymatic reaction system and studied the participation of key enzymes involvement in resveratrol production. Liquid enzyme extract were prepared from Aspergillus niger VVE1, an endophytic fungus from Cayratia trifolia plant. The observed concentration of different enzymes phenylalanine ammonia lyase (PAL), Cinnamate 4–hydroxylase (C4H) and 4–coumarate–CoA ligase (4CL) 102.34±25, 61.78±1.23 and 298.54±1.77 U/mg protein. The production of resveratrol in the presence of these enzymes was confirmed by thin layer chromatography (TLC) and High performance liquid chromatography (HPLC).

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Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

STUDY OF ENZYME ACTIVITY FOR RESVERATROL PRODUCTION BY ENDOPHYTIC FUNGUS ASPERGILLUS NIGER VVE1, Chetana Roat, Meenu Saraf INDIAN JOURNAL OF APPLIED RESEARCH : Volume-9 | Issue-1 | January-2019

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