Survival studies on genetically modified bacterins  and their immunization effect on gold fish (Carassius  auratus)

S. Jeeva; J. Raja Brindha; N. C. J. Packia Lekshmi; A. Vasudevan

Volume : III, Issue : XI, November - 2013

Survival studies on genetically modified bacterins and their immunization effect on gold fish (Carassius auratus)

S. Jeeva, J. Raja Brindha, N. C. J. Packia Lekshmi, A. Vasudevan

Abstract :

Aeromonas hydrophila was known to produce hemorrhagic ulcerative disease in gold fish and causing serious losses in ornamental fish farming. The pathogenic A. hydrophila was isolated from infected gold fish (Carassius auratus) and the LD50 was determined. Four ampicillin sensitive bacterins were developed by treating the organism with heat, UV, pH and formalin. The immune responses of these four bacterins were determined by injecting in fish individually and also by mixing it with Freund’s complete adjuvant. High levels of agglutinating antibody titers were formed in adjuvant mixed vaccinated fish and significant protection was observed in the fish when they were experimentally challenged. The survival study of these mutant strains in aquatic environment clearly indicate that the mutant strains decline rapidly suggesting that nutrient supplementation was needed for the survival of those genetically modified ampicillin mutants.