Synergistic hepatoameliorative potential of silymarin and ethanolic extract of jasonia montana versus bisphenol induced hepatotoxicity in rats

Abd Elrehim. A. Elghannam; Hoda I. Bahr; Asmaa. E. Abou El–Ftouh

Volume : VII, Issue : VIII, August - 2017

Synergistic hepatoameliorative potential of silymarin and ethanolic extract of jasonia montana versus bisphenol induced hepatotoxicity in rats

Abd Elrehim. A. Elghannam, Hoda I. Bahr, Asmaa. E. Abou El Ftouh

Abstract :

The current work is designed to explore the hepatoameliorative impacts of silymarin and ethanolic extract of jasonia montana versus bisphenol A (BPA) induced hepatotoxicity in a time dependent manner. Rats were allocated into five groups as follows; Group i: rats orally received Propylene glycol (1ml/kg B.W/day), served as control. Groups ii: rats orally received Propylene glycol for 2 weeks, then received BPA (50 mg/kg) in Propylene glycol. Groups iii–v: rats orally received (Silymarin, Jasonia Montana, both herbs) (200 mg/kg bw,150 mg/kg B.W/day, both doses) respectively for 2 weeks, then administered BPA in combination with herbs for 6 weeks. BPA induced hepatotoxicity as confirmed by increased liver enzyme leakage, hepatic 4–HNE, reduced TAC activity, IGF–1 and GSH–PX, GST gene expression. Further, BPA evoked apoptotic effect as expressed by high hepatic caspase–3 activity, TNF–α level with lower BCL–2 level. As well as fiotic effect as indicated by increased hepatic TGF–B1 and confirmed by histopathological investigation. On contrary, oral Silymarin and ethanolic extract of jasonia montana supplementation attenuated bisphenol–A evoked alterations in the above–mentioned markers all the experimental periods specially after 8 weeks, meanwhile both herps don’t affect GSH–PX, GST gene expression. Taken together, the present results summarize that J. Montana ethanolic extract supplementation showed hepatoprotctive effects versus bisphenol– induced hepatic toxicity, while silymarin can be served as a novel hepatoprotective medication and combination of both herps was better than each alone due to their synergistic antioxidant properties.