Synthetic Dye Decolourization Activity of Laccase  Enzyme: A Novel Approach to Brawl Against  Environment Pollution

Ashok V. Gomashe; Preeti G. Dharmik

Volume : IV, Issue : VI, June - 2014

Synthetic Dye Decolourization Activity of Laccase Enzyme: A Novel Approach to Brawl Against Environment Pollution

Ashok V. Gomashe, Preeti G. Dharmik

Abstract :

A research was carried out on production of laccases by fungi isolated from soil. Decolorization of two synthetic dyes (Reactive Black 5 and Reactive Blue 171) using three sources of fungal laccase with the origin of Rhizopus oryzae, Aspergillus flavus and Fusarium sporotrichioides was investigated. Among them, the enzyme from Rhizopus oyzae was found to the most efficient which decolorized 70% RB5 and 64% RB171 followed by Fusarium sporotrichioides and Aspergillus flavus. All conditions used were important for dye decolorizing activity of this crude laccase enzyme. The optimum pH of decolorization was tested at 30 °C and it was around 3.0. This activity was highly reduced at pH 4.5–5.0. This crude laccase could also decolorize the mixed dyes (Reactive Black 5 and Reactive Blue 171) and synthetic wastewaters. Rapid and high textile dye decolourization through the selection of appropriate conditions could facilitate the development of more economical and environmentally friendly processes.