IJAR - Indian Journal of Applied Research

Naresh Chand Sharma; Dhirendra Kumar; Preety Sinha; Devinder Kumar Seth; Sushil Kumar Gupta

Volume : V, Issue : III, March - 2015

Tetracycline Resistant Vibrio Cholerae O1 Ogawa With Ctxb7 Allele (Haitian) is Prevalent in Haryana, India

Naresh Chand Sharma, Dhirendra Kumar, Preety Sinha, Devinder Kumar Seth, Sushil Kumar Gupta

Abstract :

Assessment of antimicrobial resistance and cryptic changes in the ctxB gene in Viio cholerae O1 are important for the surveillance and better management of cholera disease. The present study was undertaken to assess the resistance of tetracycline and variation in ctxB of V. cholerae O1 isolated from different parts of Haryana. Bacterial characterisation was done using biochemical and serological assays. Double mismatch amplification mutation assay (DMAMA) PCR was used for the detection of ctxB7 (Haitian genotype). All isolates were identified as V. cholerae O1 biotype El Tor serotype Ogawa. DMAMA PCR assays confirmed the presence of ctxB7 Haitian genotype in all the isolates. These isolates were multidrug resistant including tetracycline. V. cholerae isolates were commonly resistant to nalidixic acid, co–trimoxazole and tetracycline in this region and a new variant of ctxB was also identified.