Volume : V, Issue : IV, April - 2015

The effect of in situ gene therapy of tumor in pregnant mice.

Saad Akram Hatif, Aysar Saadoon Abbood

Abstract :

 The aim of study to investigation the side effect of gene therapy in mice by the local injection of ( DNA 

with RNA of camel ) in the mice were adinoarcinoma infected, ( 30 mice were treated ,10 control and 6 
healthy males ) . The method of Chloroform –phenol was utilized for extracted the nucleic acid from liver of camel 
. Treated mice were classified into two groups, The first group (15 mice ) subjected for local injection in the tumor 
while the second group(15 mice) treated by intramuscular injection. Treated females mixed with the male in order to 
conception. The control group and the second group were dead during 5–14 day according to the severity of case 
, while (13 mice ) at the first group has recovered when intra tumor injection of camel nucleic acid. Survived mice 
completed pregnancy subsequent by ed normal fetuses without abnormality, the birth number ranged between 5–7 
born for every mice , new birth leave up to adulthood. Consequently the new generation leave with the adult male 
for conception and fertility. Another generation of pregnant mice has ed normal fetuses without abnormalities. In 
conclusion the direct injected of (DNA with RNA of camel) in the adenocarcinoma more efficient than in the intramuscular injection, in addition, no complications in the pregnant mice or fetuses , means the direct injection of genes lead 
to mortality of cell tumor. 

Keywords :

Article: Download PDF   DOI : 10.36106/ijar  

Cite This Article:

Saad Akram Hatif, Aysar Saadoon Abbood The effect of in situ gene therapy of tumor in pregnant mice. Indian Journal of Applied Research, Vol.5, Issue : 4 April 2015


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