Thermophilic Lipase from Lysinibacillus mangiferihumi: Screening and partial characterization

Tambekar DH; SD Tambekar; Jadhav AS; Sharma NH

Volume : VI, Issue : VI, June - 2016

Thermophilic Lipase from Lysinibacillus mangiferihumi: Screening and partial characterization

Tambekar Dh, Sd Tambekar, Jadhav As, Sharma Nh

Abstract :

 Alkaline Lonar Lake, a unique ecosystem situated in Buldhana District of Maharashtra State, India, harbors various haloalkaliphilic bacterial species which produces biotechnologically important thermo–haloalkaliphilic enzymes such as lipase. Lipases are diversified enzymes in their properties and substrate specificity, which make them attractive tools for various industrial applications. In this study, an alkalinethermostable lipase producing bacterium was isolated from Lonar Lake and ccharacterized morphologically, culturally and biochemically and identified as Lysinibacillus mangiferihumi by 16S rRNA sequencing. Alkaline lipase production was optimum at pH 9 and at 600C and enzyme activity was maximum at 1.54 unit/mL to 1.66 unit/mL. Lipase from this bacterium was active at higher temperature and pH and finds potential applications in food, pharmaceutical and detergent industries.