Utility of MGIT�960 for detection of Mycobacterium tuberculosis in extrapulmonary specimens.

Sumit Goyal; Bharti Malhotra; Shipra Bhargava; Deepika Verma

Volume : V, Issue : XI, November - 2015

Utility of MGIT�960 for detection of Mycobacterium tuberculosis in extrapulmonary specimens.

Sumit Goyal, Bharti Malhotra, Shipra Bhargava, Deepika Verma

Abstract :

 Background and Objective: Extra Pulmonary Tuberculosis (EP–TB) contributes 15 to 20% cases of M. tuberculosis (MTB) in India and due to paucibacillary nature, TB often remains undiagnosed. The aim of present study was rapid diagnosis of MTB in EP samples by using Mycobacteria Growth Indicator Tube (MGIT) 960. Methods and Material: A total of 101 EP specimens were processed for Acid–Fast Bacilli (AFB) smear microscopy and culture by MGIT 960. MTB and Non Tuberculous Mycobacteria (NTM) were further differentiated by SD Bioline TB Ag MPT 64 rapid assay on MGIT positive specimens. Results: Out of 101 specimens, 2 (1.98%) were found smear positive, while 18 (17.82%) were positive on MGIT–960 culture. Amongst 18 positive isolates, all isolates were MTB (100%). Smear positive samples were detected on an average in 14.1 days, while smear negative samples were detected in average 18.66 days. Interpretation and conclusions: Mycobacteria tuberculosis was detected in 17.82% of the total EP cases in an average of 18 days. None of the sample was positive for NTM. MGIT 960 is a good system for detection of MTB with low turn around time. The growth obtained can be further used for Drug Susceptibility Testing (DST) also.