Utility of p63 and AMACR applied as double simultaneous immunostains in diagnostic assessment of prostate specimens

Dr Ajit Nambiar; Amrutha PB; Anu Catherine Jacob

Volume : IX, Issue : I, January - 2019

Utility of p63 and AMACR applied as double simultaneous immunostains in diagnostic assessment of prostate specimens

Dr Ajit Nambiar, Amrutha Pb, Anu Catherine Jacob

Abstract :

The diagnostic assessment of problematic prostatic pathology is supported by immunohistochemistry. The loss of tissue on further sectioning of paraffin block, can be addressed by double or multiplex immunostains on a single tissue section. We evaluated in this study the utility of double immunostains of p63 and AMACR in diagnostic assessment of prostatic specimens. The absence of p63 basal marker in 100% along with intense diffuse cytoplasmic or apical expression of AMACR in 88% was noted. The overall success of 98% with double immunostains in our laboratory illustrates that it is a simple assay to perform and is an appropriate advancement in the molecular era with every bit of tissue is precious.