VALIDATION OF SIGNIFICANT PROTEIN MARKERS FROM THE PLASMA OF SEVERE DENGUE INFECTED ADULT PATIENTS USING SELECTED REACTION MONITORING ASSAY

Tundwal Vijay Kumar; Somveer; Satyaveer Singh; Gunhawat Manisha; Gahlot Narendra Kumar; Kochar Aditya; Tundwal Divyanshi; Kochar Sanjay Kumar

Volume : X, Issue : VIII, August - 2020

VALIDATION OF SIGNIFICANT PROTEIN MARKERS FROM THE PLASMA OF SEVERE DENGUE INFECTED ADULT PATIENTS USING SELECTED REACTION MONITORING ASSAY

Tundwal Vijay Kumar, Somveer, Satyaveer Singh, Gunhawat Manisha, Gahlot Narendra Kumar, Kochar Aditya, Tundwal Divyanshi, Kochar Sanjay Kumar

Abstract :

Background :Infection with dengue virus (DENV) causes a spectrum of clinical manifestations ranging from mild dengue fever (DF) to the potentially lethal dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). Dengue is endemic to the tropical and sub–tropical regions of the world, which are home to over half the population of the world as well as being popular tourist destinations Aim :To study about information of the pathogenesis and clinical profile of dengue virus and Validation of significant protein markers of dengue infected adult patients using selected reaction monitoring assays. Material &Methods :This study was conducted in Department of Medicine, Sardar Patel Medical College, Bikaner from July 2017 to December 2018. Total 26 cases with features of dengue illness along with positive dengue serology in this duration were admitted in hospital. This was a hospital based study. Results : Proteomic analysis in severe dengue patients (DHF/DSS) shows down regulation of three significant proteins CD–44 antigen, complement component C8 beta chain and leucine–rich alpha–2–glycoprotein as compared to healthy controls. According to dengue agglutination test, 100% cases had IgM positive while 69.2% cases had NS1 positive. No case had IgG positive. Mean age in DHF group was 30.4812.58 and in DSS group mean age was 25.0012.39 years and this difference was found statistically insignificant (p>0.05). Most common clinical manifestation was fever, abdominal pain and myalgia (100%) while rash was present in 96.2%, vomiting and headache were present in 92.3% cases each and least common clinical manifestation was retro–orbital pain (88.5%).Gum bleeding, epistaxis and petechiae were found in most cases.According to sensorium 96.2% cases were found normal and 3.8% cases were altered sensorium.According to outcome, 20 cases were cured successfully while 6 cases discharge on request. Patients were on telephonically follow up for one week, there was no mortality. Conclusion : In present study revealed that validating 26 serum samples used for optimization by using selected reaction monitoring. Three significant proteins, CD44 Antigen, Complement component C8 beta chain, Leucine–rich alpha–2–glycoprotein are downregulated in severe dengue (DHF/DSS) patients as compared to healthy controls. These are predictive biomarkers of severe dengue fever (DHF/DSS).