IJSR - International Journal of Scientific Research

Babu R L; Vijayalakshmi E; Naveen Kumar M; Rajeshwari H. Patil; S Chidananda Sharma

Volume : II, Issue : VIII, August - 2013

Blocking of CDK4 Gene Expression by Antisense Oligos Induce Apoptosis and Decreases the Proliferation of A549 Cells

Babu R L, Vijayalakshmi E, Naveen Kumar M, Rajeshwari H. Patil, S Chidananda Sharma

Abstract :

Cell cycle is a highly regulated process and can be oadly divided into G1, S, G2 and M phases. Cyclins Ds interact and activate cyclin dependent kinases CDK4/6. CDKs are also controlled by family of CDK inhibitory proteins. CDK4 is expressed in a variety of normal cells, but over expressed in human tumours. Blocking of CDK4 expression could be an attractive strategy for the treatment of cancers. Hence, in the present study CDK4-antisense oligos were used for the blocking of CDK4 expression in A549 cells. A549 cells transfected with CDK4-antisense oligos inhibit the proliferation of cells in a dose dependent manner and show increased expression of a pro-apoptotic Bax gene by 35%, and caspase-8 by 3 folds. Further, reduction in the expression of cyclin D1 and anti-apoptotic Bcl-2, suggested that the blocking of CDK4 expression in A549 cells inhibit the cell cycle with induced apoptosis.