Effect of Follic acid Antagonist Methotrexate (MTX) on the level of testosterone of Funambulus pennanti (wroughton)

S.R. Kohchale

Volume : IX, Issue : IV, April - 2020

Effect of Follic acid Antagonist Methotrexate (MTX) on the level of testosterone of Funambulus pennanti (wroughton)

S. R. Kohchale

Abstract :

Effect of Methotrexate on the level of testosterone have been studied by intramuscularly injecting low dose of 3 mg/kg BW/ per day and 6 mg/kg BW/ day for 15 days to adult male squirrel (Funambulus pennanti) during the eeding period January. For comp� the effects saline treated vehicle was injected same amount of saline and were maintained for the same duration. Level of testosterone was determined by Enzyme Linked Fluorescent Essay (ELFA), significant decrease in the testosterone level after high dose treatment and insignificant decrease after low dose treatment observed. From the foregoing it is concluded that Methotrexate has adverse effect on spermatogenesis which are dose and duration dependent besides being toxic, therefore certainly causing reduction in the fertility rate