IJSR - International Journal of Scientific Research

RM VATCHALARANI; ROHIT SHARMA; MANAS BAJPAI; POOJA GUPTA; MANJUNATH BC

Volume : VIII, Issue : VIII, August - 2019

ROLE OF CONNECTIVE TISSUE IN AFFECTING THE NATURE OF OVERLYING EPITHELIUM IN VARIOUS ODONTOGENIC CYSTS – A POLARIZING MICROSCOPIC STUDY.

Rm Vatchalarani, Rohit Sharma, Manas Bajpai, Pooja Gupta, Manjunath Bc

Abstract :

Background: Connective tissue often plays an important role in the pathogenesis of odontogenic cysts and there is a necessity to understand the biological behavior of these lesions. Aims and Objectives: To evaluate the nature of collagen in connective tissue walls of keratocystic odontogenic tumor (KCOT), dentigerous cyst and radicular cyst and compare it with dental follicle. Materials and methods: A retrospective study was conducted in which 52 histologically diagnosed slides comprising of keratocystic odontogenic tumors (15), dentigerous cysts (15), radicular cysts (15), normal mucosa (2) and dental follicular tissue (5) were stained with picrosirius red and observed under polarized microscope. The interpretations of the color profiles exhibited by the collagen fibers given by Junqueira et al., was considered as standards to interpret the tissue sections. The obtained data were analyzed using SPSS (Statistical Package for the Social Sciences) for Windows (Version 18.0), and Pearson chi–square test was used with statistical significance fixed at 0.05. Results: Greenish yellow birefringence (GY) was recorded more in keratocystic odontogenic tumor (46.7%) when compared to radicular cyst (13.3%) which was highly significant (P value .088). Reddish orange (RO) was more among the samples of radicular cyst (40%), Greenish yellow birefringence (GY) was predominantly in Dental follicle (60%), and Yellowish orange (YO) was predominantly observed in buccal mucosa (50%). Conclusion: The appearance of collagen fies in the keratocystic odontogenic tumor was predominantly different suggesting that connective tissue may be responsible for the biologic behavior of the cysts